Title: 5.1 Developing a Prototype
Course: ATEP - Biotechnology Module B > Section: Section 5 Developing a Prototype

5.1.1 Recap

By now you should have worked through the first four steps of the Informed Design process. Discuss with your team how each one of these steps in the design process helped you in the bioreactor design. Be sure to address each step by name and give a specific example related to your bioreactor design.

5.1.2 Developing a Prototype

You are now ready to tackle the next step of the design process: developing a prototype. Using your Knowledge Skill Builder worksheets from Lesson 2.1, you will now begin to build your bioreactor. The image below is a good starting point.

Generate an SOP for building your bioreactor. Your instructor must approve your SOP before building. Return to lesson 1.4 to review the parts of an SOP.

5.1.3 Construction Day!

Using your teacher approved SOP construct your bioreactor and upload a picture of your prototype to the discussion board below.

5.1.4 Sterilization

Anything that will be used to grow your culture must be sterilized first. Below are steps to follow for sterilization using diluted bleach.

1. Always wear gloves.
2. Using a paper towel wipe down your bench with diluted bleach.
3. Fill an empty beaker with isopropyl alcohol. Insert forceps to soak in beaker for at least 15 minutes.
4. Fill a bucket with 10% bleach and submerge cap and open bottle for 10 minutes.
5. Drain the bleach solution.
6. Rinse the bottle and cap three times with cool sterile water.
7. Cap the bottle. Seal the sampling tube by folding it back over itself and attaching a clip to keep it closed.
8. Use the sterile forceps to stuff the sterile cotton wool lightly into the end of the exhaust tube and the aeration tube. Connect the aeration tube to the air pump.