Title: Default for ATEP-BIO-B
Course: ATEP - Biotechnology Module B

Q: Which of these is not a general rule in the lab?
- 1. Use goggles, lab apron, face mask, and appropriate gloves when instructed
- 1. Never smell a material in a test tube or flask directly
- 1. Never take chemical stock bottles to the lab bench
- 1. All of the above are rules
A: 1. All of the above are rules

Q: Which of these is not a general rule in the lab?
- Food and drink are allowed in the lab as long as they are not consumed at the lab bench.
- Never taste any materials in the lab.
- Report all accidents to the teacher.
- All of the above are rules
A: Food and drink are allowed in the lab as long as they are not consumed at the lab bench.

Q: When dealing with hazardous materials, security is important.  Which of the following does not need to be locked up?
- controlled substances
- balances
- computers
- MSDS forms
A: MSDS forms

Q: Which of the following is not a guideline for securing hazardous materials?
- Control access to the area.
- Know who is in your laboratory.
- Allow people you know to remove hazardous material if they promise to bring it back.
- All of the above are guidelines.
A: Allow people you know to remove hazardous material if they promise to bring it back.

Q: Which of the following is not considered a biohazard?
- live animals if they can release toxins
- sodium chloride
- recombinant DNA
- All of the above are biohazards.
A: sodium chloride

Q: Disinfectants kill ________________.
- only pathogenic bacteria
- only certain types of bacteria
- every single bacterial cell
- only rod-shaped bacteria
A: only certain types of bacteria

Q: What does MSDS stand for?
- Material Safety Data Sheets
- Mammal Safety Data Sheets
- Math Starts During Seventh
- Material Serves Data Sheets
A: Material Safety Data Sheets

Q: What does an MSDS form tell you?
- how much chemical to use
- describes the specific properties of the chemical
- how much chemical is in the container
- how old the chemical is
A: describes the specific properties of the chemical

Q: 1. There are how many sections of the MSDS?
- 1
- 3
- 5
- 8
A: 8

Q: What does Blue and Red mean respectively in the safety diamond?
- health hazard, flammability hazard
- flammability hazard, health hazard
- cold hazard, hot hazard
- hot hazard, cold hazard
A: health hazard, flammability hazard

Q: What should you do with broken glass?
- Pick it up and throw it in the trashcan.
- Place it in the broken glass disposal box.
- Leave it.
- Ask your teacher to throw it away.
A: Place it in the broken glass disposal box.

Q: What is the BEST way you mark glassware?
- Use pen or permanent mark on the white marking area.
- Use expo marker on the white marking area.
- Place a piece of tape on the glassware for writing.
- Place a marker on the underside of the glass.
A: Place a piece of tape on the glassware for writing.

Q: A label should always contain ____________.
- the contents of the container
- your initials and class period
- date
- the color of the solution
A: the color of the solution

Q: What are beakers used for?
- measuring
- stirring, mixing, and heating
- storing chemicals
- large amounts over 1 liter
A: stirring, mixing, and heating

Q: Which of the below would you use a graduated cylinder for in the laboratory?
- measuring liquids
- stirring, mixing, and heating
- storing chemicals
- measuring solids
A: measuring liquids

Q: Which of the below would you use a transfer pipette for in a laboratory?
- transfer small amount of liquid (1-2 ml)
- transfer solids less than 10 grams
- transfer large amounts of liquid (50-100ml)
- measure amounts greater than 50 ml
A: transfer small amount of liquid (1-2 ml)

Q: How will you measure the mass in the labroratory?
- bathroom scale
- triple beam balance
- electric pump
- Bunsen burner
A: triple beam balance

Q: What is the difference between a triple beam balance and an electronic balance?
- The electronic balance is more precise.
- The electronic balance is less precise.
- The electronic balance measures weight.
- The electronic balance measures mass.
A: The electronic balance is more precise.

Q: What does agar do for the bacteria?
- gives them comfort to grow on
- gives them water
- gives them shelter
- gives them food
A: gives them food

Q: 1. What does each dot on your agar represent?
- one group of cells
- one individual cell cloned to be many
- similar cells but not identical
- a single protein
A: one individual cell cloned to be many

Q: Which of the following items would be appropriate entries into an engineering notebook? (Choose all that are correct.)
- speed = distance/time
- [Image]
- Today, my team members and I decided to play Wordle.
- [Image]
- constraint of our design model
A: speed = distance/time; [Image]; [Image]; constraint of our design model

Q: Entries into your engineering notebook should: (Choose all that are true.)
- be written in ink
- include blank pages
- be taped on all four corners if entering a picture
- be signed and dated
- be erased or whited out of incorrect entries are made
- title at the top of the page
- listed in the table of content
- only be computer generated
A: be written in ink; be taped on all four corners if entering a picture; be signed and dated; title at the top of the page; listed in the table of content

Q: Use the Internet to research the statement below and discover if they are true statements that led to increased federal regulations. Choose all false statement below:

1. In 1955, 51 people were paralyzed and 10 died from the polio vaccine due to a manufacturer who did not properly inactivate the virus.
2. In 2002, insulin produced by recombinant DNA methods was the first biotechnology produced pharmaceutical product to be approved for market.
3. Before the year 1927, there were no regulations in place by the federal government to ensure the safety and effectiveness of drugs.
4. In the early, 1960s the drug thalidomide was prescribed for nausea in pregnant woman and resulted in the birth of thousands of children without arms and legs.
- Statement 1
- Statement 2
- Statement 3
- Statement 4
A: Statement 2

Q: Using an SOP from the tech park, select all of the sections that are present in the document
- materials
- scope
- reference
- Statement of Purpose
- Statement of Authority
A: materials; scope; reference; Statement of Purpose

Q: For which of the following events would you create an SOP?
- Drawing conclusions from an experiment
- Preparing nutrient agar plates
- identifying constraints and specifications of a design
- Design sketches
A: Preparing nutrient agar plates

Q: For which of the following events would you create an SOP?
- drawing conclusions from an experiment
- preparing nutrient agar plates
- identifying constraints and specifications of a design
- design sketches
A: preparing nutrient agar plates

Q: What safety precautions will you to take to ensure that the bioreactor does not explode?
- Heat the bottle up to 95 degrees Fahrenheit.
- Keep the bioreactor in an incubator.
- Vent the bioreactor.
- Be sure there are no vents on the bioreactor.
A: Vent the bioreactor.

Q: Which organisms will be used in your bioreactor?
- lactobacillus
- E. coli
- yeast
- lactase
A: lactobacillus

Q: Where will the original inoculation of Lactobacillus come from?
- a science supply company
- a yogurt sample
- a pure probiotic sample
- milk
A: a pure probiotic sample

Q: What enzyme will you be trying to mass produce using Lactobacillus?
- lactid acid
- protease
- milk
- lactase
A: protease

Q: Which of the following could be used as a food source for Lactobacillus? Choose all that are correct.
- sugar
- powdered milk
- NaOH
- tums
A: sugar; powdered milk

Q: How long after inoculation would you expect to have maximum protease (product) secreted by the Lactobacillus?
- 1 hour
- 24 hours
- 10 hours
- 48 hours
A: 10 hours

Q: Which component below could you add to the medium to increase Lactobacillus growth?
- NaOH
- HCl
- base
- distilled water
A: HCl

Q: Select the conditions below that would allow for maximum growth of Lactobacillus:
- pH 3
- pH 9
- 37 degrees Celcius
- 98 degrees Celsius
- absence of oxygen
A: pH 3; 37 degrees Celcius

Q: Bacteria growth over time can be measured by
- autoclaving the sample.
- plating the sample on agar plates and counting colonies.
- counting each cell viewed under a light microscope.
- leaving sample untouched fro at least 78 hours.
A: plating the sample on agar plates and counting colonies.

Q: Protease production will be measured using a(n)
- gelatinase test.
- hydrogen peroxide test.
- counting colonies on an agar plate.
- using a turbidity scale.
A: gelatinase test.

Q: Your bioreactor design should include _____ holes.
- one
- two
- three
- four
A: three

Q: Openings leading into or out of the bioreactor can be kept fairly sterile by stuffing it with
- sterile cotton.
- air beads.
- tape.
- sterile gloves.
A: sterile cotton.

Q: Which of the following would be the best choice for a growth chamber for your bioreactor?
- a plastic two-liter bottle
- an aluminum can
- a sealed graduated cylinder
- an agar plate
A: a plastic two-liter bottle

Q: The inlet tubing should be attached towards the ______ of the bioreactor.
- top
- middle
- bottom
A: top

Q: if your classroom lacks an autoclave your may soak all bioreactor pieces in ________.
- 10% bleach solution
- dishwashing liquid
- distilled water
- 75% bleach solution
A: 10% bleach solution

Q: Lactobacillus will be grown on ________ agar plates to isolate bacteria.
- LMRS
- fructose
- agarose
- glucose
A: LMRS

Q: The purpose of diluting a bacteria sample before adding it to an agar plate is
- to produce an agar plate with a countable number of isolated colonies.
- to produce a lawn of bacteria on the agar plate.
- to produce an agar plate with 5-10 isolated colonies.
- to produce more protease.
A: to produce an agar plate with a countable number of isolated colonies.

Q: The more protease that is produced and secreted into the environment,
- the faster gelatin will liquify.
- the slower gelatin will liquify.
- the more protease will decompose.
- the more gelatin will solidify.
A: the faster gelatin will liquify.

Q: The gelatin must be made _______; whereas, the protease solution must be made ________.
- a day before the experiment; on the day of the experiment
- on the day of the experiment; a day before the experiment
- a week before the experiment; a day before the experiment
- immediately before performing the experiment; three hours before
A: a day before the experiment; on the day of the experiment

Q: What equipment will you use to measure 7.1 g of gelatin?
- triple beam balance/electric balance
- graduated cylinder
- 1 liter flask
- a beaker
A: triple beam balance/electric balance

Q: What equipment will you use to measure 500 ml of distilled water?
- a 1000-L beaker
- a 1000-ml graduated cylinder
- a 500-ml beaker
- a 200-ml graduated cylinder
A: a 1000-ml graduated cylinder

Q: In the protease assay, the purpose of the second tube that contains meat tenderizer is
- to create a positive control.
- to create a negative control.
- to stop protease activity.
- to increase protease activity.
A: to create a positive control.

Q: Adding methylene blue to a bacteria sample allows the bacteria
- to be visible under the light microscope.
- to live longer in the absence of oxygen.
- to produce more protease.
- to grow exponentially.
A: to be visible under the light microscope.

Q: Which of the following variables can be classified as a dependent variable for your bioreactor?
- the time it takes gelatin to liquify
- room temperature
- pH 3
- turbidity
A: the time it takes gelatin to liquify

Q: What equipment will you use to heat your media solution to a boil?
- hot plate
- microwave
- oven
- hot water form the sink
A: hot plate

Q: When should you perform the first protease test?
- immediately following inoculation
- one hour after inoculation
- 24 hours after inoculation
- right before adding Lactobaccillus
A: immediately following inoculation

Q: Glassware should be marked with
- a pen
- tape
- pencil
- nothing
A: tape

Q: _Select all of the information that should be on any labeled container:
- date
- full last name
- contents
- location
A: date; contents

Q: Select the best piece of equipment to measure 10 ml:
- a beaker
- a graduated 5-ml pipette
- a graduated cylinder
- a 25-ml beaker
A: a graduated cylinder

Q: If you measure an object and it is 10 grams, what have you measured?
- mass
- volume
- density
- weight
A: mass

Q: [Image]
A: 354.6

Q: Each dot on this agar represents
- a bacterial colony.
- 300 cells.
- lactose.
- agar.
A: a bacterial colony.

Q: How many ml of water is shown below?

[Image]
A: 8.8

Q: The piece of equipment below would best measure

[Image]
- 0.5 ml
- 0.5 grams
- 50 ml
- 5 ml
A: 0.5 ml

Q: Mass will be measured in units of liters.
- True
- False
A: False

Q: Solutions will be heated using a Bunsen burner.
- True
- False
A: False