Course Change

Section I: BASIC COURSE INFORMATION

Outline Status: Approved Outline

1. COLLEGE: L.A. MISSION COLLEGE

2. SUBJECT: BIOTECHNOLOGY

3. COURSE NUMBER: 003

4. COURSE TITLE: BIOTECHNOLOGY II

5. UNITS: 4

6. CATALOG COURSE DESCRIPTION:

This course expands concepts and techniques introduced in Biotechnology I. Students are introduced to modern molecular biology techniques, including nucleic acid isolation, recombinant DNA techniques, cell transformation, recombinant DNA analysis, nucleic acid hybridization, and DNA sequence analysis. Students explore the production and purification of proteins using biochemical techniques such as immunochemistry and chromatography.

7. CLASS SCHEDULE COURSE DESCRIPTION:

8. INITIAL COLLEGE APPROVAL DATE: 10/4/16

9. LAST UPDATE DATE: 10/4/16

10. CLASS HOURS:

	Standard Hrs Per Week (based On 18 weeks)		Total Hs per Term (hrs per week x 18)		Units
Lecture:	2		36		2
Lab/Activity (w / homework):	0		0		0
Lab/Activity (w /o homework):	6		108		2
Totals:	Lecture:	2	Lecture:	36	Lecture:	2
	Lab:	6	Lab:	108	Lab:	2
	Total:	2	Total:	36	Total:	4

Totals In Protocol:	Lecture:	2	Lecture:	36
	Lab:	6	Lab:	108
	Total:	8	Total:	144	Total:	4

11. PREREQUISITES, COREQUISITES, ADVISORIES ON RECOMMENDED PREPARATION, and LIMITATION ON ENROLLMENT:

Note: The LACCD's Policy on Prerequisites, Corequisites and Advisories requires that the curriculum committee take a separate action verifying that a course's prerequisite, corequisite or advisory is an 'appropriate and rational measure of a student's readiness to enter the course or program' and that the prerequisite, corequisite or advisory meets the level of scrutiny delineated in the policy.

PREREQUISITES: Yes

	Subject	Number	Course Title	Units	Validation Approval Date
	BIOTECHNOLOGY	002	Biotechnology I	3	10/4/16

COREQUISITES: No

Subject

Number

Course Title

Units

Validation Approval Date

ADVISORIES: No

Subject

Number

Course Title

Units

Validation Approval Date

12. OTHER LIMITATIONS ON ENROLLMENT: (See Title 5, Section 58106 and Board Rule 8603 for policy on allowable limitations. Other appropriate statutory or regulatory requirements may also apply):

Section II: COURSE CONTENT AND OBJECTIVES

1. COURSE CONTENT AND OBJECTIVES:

COURSE CONTENT AND SCOPE - Lecture: Outline the topics included in the lecture portion of the course (Outline reflects course description, all topics covered in class).	Hours per topic	COURSE OBJECTIVES - Lecture: Upon successful completion of this course, the student will be able to..(Use action verbs - see Bloom's Taxonomy for 'action verbs requiring cognitive outcomes.')
1. Biotechnology Overview	1	1. Describe the range of biotechnical skills to be acquired in this course and their uses in research and industry.
2. Restriction Enzymes	2	2. Analyze the function and variety of restriction endonucleases and their uses in biotechnology.
3. RNA Purification	2	3. Discuss the different functional types of RNA, methods of RNA purification, and explain how RNA degradation can be avoided.
4. cDNA Synthesis	2	4. Explain how cDNA is synthesized from RNA and its role in biotechnology.
5. DNA Purification	2	5. Compare the characteristics of DNA from prokaryotic and eukaryotic cells, viruses, and extrachromosal DNA structures such as plasmids, and methods used to purify each type of DNA.
6. DNA Sequencing	2	6. Explain the various methods of DNA sequencing and the uses for DNA sequence information.
7.Polymerase Chain Reaction (PCR)	3	7. Explain the process of PCR amplification of DNA, the role of each component in a PCR reaction, and the uses of this technique in biotechnology.
8. RT-PCR	1	8. Discuss the process of RT-PCR and its roles in biotechnology.
9. Quantitative PCR	1	9. Explain how qPCR reveals the quantitative expression of a gene.
10. DNA Cloning	3	10. Distinguish the processes of cloning genomic DNA and cDNA fragments and their applications in biotechnology.
11. Protein Expression Systems	2	11. Compare various commonly used protein expression systems and the advantages/disadvantages of each.
12. Protein Purification	2	12. Explain the various methods used to purify specific proteins from a complex mixture.
13. High Performance Liquid Chromatography (HPLC)	2	13. Explain how HPLC is used to purify a molecule from a complex mixture.
14. Antibodies	2	14. Distinguish polyclonal and monoclonal antibody production and how each are used to identify or purify proteins.
15. Western Blotting	1	15. Explain the process of performing a western blot and how this technique is used to identify specific proteins.
16. Enzyme Linked Immunosorbant Assay (ELISA)	1	16. Distinguish indirect ELISA from direct ELISA and explain how these techniques are used to identify the presence of specific proteins or antibodies in a test sample.
17. Immunohistochemistry	1	17. Explain how labeled antibodies are used to identify specific proteins in cell and tissue samples.
18. Mammalian Cell Culture	4	18. Discuss the chemical and environmental conditions required to successfully culture mammalian cells and avoid microbial contamination.
19. Good Manufacturing Practices (GMPs)	2	19. Explain the characteristics of good manufacturing practices and their importance in biotechnology.

Total:	36

Total Hrs In Protocol:	36

1. (cont'd) LAB:

COURSE CONTENT AND SCOPE - Lab: Outline the topics included in the laboratory portion of the course (Outline reflects course description, all topics covered in class).	Hours per topic	COURSE OBJECTIVES - Lab: Upon successful completion of this course, the student will be able to..(Use action verbs - see Bloom's Taxonomy for 'action verbs requiring cognitive outcomes.')
1. Restriction Enzyme Analysis	7	1. Plan and carry out restriction enzyme digestions that are analyzed by agarose gel electrophoresis, determine the size of DNA fragments on the gel, construct a simple restriction map.
5. Polymerase Chain Reaction (PCR)	7	5. Plan and carry out PCR amplification of desired DNA sequences, design PCR primers, analyze PCR products by gel electrophoresis.
6. RT-PCR	4	6. Create cDNA from previously purified RNA, PCR amplify the cDNA and analyze by gel electrophoresis.
7. DNA Cloning	10	7. Plan and prepare DNA insert and vector for ligation, and ligate them together.
8. Transformation of Bacteria	3	8. Transform bacteria with DNA ligation reaction and plate the bacteria on selective agar medium for blue/white selection.
9. Plasmid Minipreps	4	9. Carry out a small scale plasmid DNA purification from blue and white colonies of bacteria transformed with the vector/insert ligation reaction.
10. Recombinant DNA Analysis	7	10. Distinguish positive bacterial clones by planning and carrying out diagnostic restriction enzyme digestions of plasmid DNA samples analyzed by agarose gel electrophoresis.
11. Protein Purification	11	11. Purify proteins from a complex mixture using a variety of common techniques such as HPLC, immunoprecipitation, and column chromatography.
2. RNA Purification and cDNA Synthesis	7	2. Purify total RNA and mRNA from cells and tissues and synthesize cDNA from the mRNA.
3. DNA Purification	6	3. Purify chromosomal and plasmid DNA from bacteria and purify genomic DNA from mammalian cells or tissues.
4. Bioinformatics	5	4. Analyze DNA sequence information and online bioinformatics resources to find open reading frames and homologous sequences in various databases.
12. SDS-PAGE	4	12. Prepare and run SDS-PAGE to analyze purified protein samples.
13. Western Blotting	7	13. Plan and carryout SDS-PAGE followed by a western blot to determine the presence and molecular weight of specific proteins within a complex mixture.
14. Immunohistochemistry	7	14. Apply the process of immunohistochemistry to identify the expression of specific proteins within tissue or cell samples.
15. Enzyme Linked Immunosorbent Assay (ELISA)	7	15. Analyze complex samples to identify the presence of specific antibody or antigen in a test sample using the ELISA technique.
16. Mammalian Cell Culture	9	16. Prepare sterile media and aseptically culture a variety of mammalian cell lines, determine cell counts using a hemocytometer.
17. Good Manufacturing Practices (GMPs)	3	17. Explain and apply good manufacturing practices in a biotechnology laboratory environment.

Total:	108

Total Hrs In Protocol:	108

1. (cont'd) SLO:

Outcome - The student will: (Describe the major outcomes that a successful student will gain from the class for use in his/her life. Use higher order Bloom's taxonomy verbs.)	1. Explore the production and purification of recombinant proteins using biochemical techniques such as immunochemistry and chromatography. . 2. Examine and apply complex molecular biology techniques to clone a gene fragment. Benchmark - 75% of the students will achieve a rubric score of acceptable or better.
Assessment - as measured by the following method: (Please indicate the criteria and rating scale by which the assessment will be evaluated.)	1. Protein purification assignment for which students are assessed for the following criteria: quantity of specific protein, purity of specific protein, and characterization of specific protein. The assessment will use a rubric designed as follows: 4 - exemplary, 3 - good, 2 - acceptable, 1 - poor, 0 - unacceptable. 2. DNA cloning project for which students are assessed for the following criteria: accuracy of calculations, PCR amplification, quality of transformation, number of positive clones. The assessment will use a rubric designed as follows: 4 - exemplary, 3 - good, 2 - acceptable, 1 - poor, 0 - unacceptable.
ILO - which Institutional Learning Outcome(s) (ILO) does this SLO support? (See College Catalog p. 10.)	Problem Solving (1, 2) Quantitative Reasoning (1, 2)

Essential Academic Skills: Reading and Communication

2. REQUIRED TEXTS:

Provide a representative list of textbooks and other required reading; include author, title and date of publication:

Methods in Biotechnology, Seung-Beom Hong, M. Bazlur Rashid, Lory Z. Santiago-Vazquez, 2017

3. READING ASSIGNMENTS:

If applicable, reading assignments in this course may include but are not limited to the following:

Assigned reading from texts, standard operating procedures.

4. WRITING ASSIGNMENTS:

Writing assignments, as required by Title 5, in this course may include, but are not limited to the following:

SOP design/writing, reports of experimental results, lab notebook, summary and analysis of guest lecture or other outside of class presentation.

Essential Academic Skills: Critical Thinking and Other Course Components

5. REPRESENTATIVE ASSIGNMENTS THAT DEMONSTRATE CRITICAL THINKING:

Provide examples of assignments, as required by Title 5, that demonstrate critical thinking.

Students will be required to analyze, evaluate and interpret experimental data and communicate this information using appropriate terminology.

6. SELF-REFLECTIVE LEARNING:

If applicable, describe how students will reflect on their development as active learners. Provide representative examples below.

7. COMPUTER COMPENTENCY:

If applicable, explain how computer competency is included in the course.

Accessing information and class resources from the internet, preparing reports and storing data.

8. INFORMATION COMPENTENCY:

If applicable, explain how information competency is included in the course.

Students will be required to evaluate, analyze and interpret experimental data. They will be required to communicate this information. Students will be able to incorporate their data to uses in biotechnology processes and their ethical implications.

Evaluation and Instruction

9. REPRESENTATIVE OUTSIDE ASSIGNMENTS (Homework):

Out of class assignments may include, but are not limited to the following:

Assigned readings from the texts and other sources such as industry specific journal articles, assigned writings, planning for oral presentations, problem sets.

10. METHODS OF EVALUATION:

Title 5, section 55002 requires grades to be 'based on demonstrated proficiency in subject matter and the ability to demonstrate that proficiency, at least in part, by means of essays, or, in courses where the curriculum committee deems them to be appropriate, by problem solving exercises or skills demonstrations by students.' Methods of evaluation may include, but are not limited to the following (please note that evaluation should measure the outcomes detailed 'Course Objectives' at the beginning of Section II):

Essays on exams, problem solving on exams, Objective exams, classroom discussion, Lab reports, projects,skill demonstration, participation.

11. METHODS OF INSTRUCTION:

Methods of instruction may include, but are not limited to the following.

Discussion
Activity
Field Experience
Independent Study
Purposeful Collaboration
Other (Please Explain)

12. SUPPLIES:

List the supplies the student must provide.

Lab coat, lab notebook, Scantrons, blue books, calculator

13. DIVERSITY:

If applicable, explain how diversity (e.g., cultural, gender, etc.) is included in the course.

Does not meet diversity requirements

13. SCANS COMPETENCIES:

(required for all courses with vocational TOP Codes; recommended for all courses)

SCANS (Secretary's Commission on Necessary Skills) are skills the Department of Labor identified, in consultation with business and industry leaders, which reflect the skills necessary for success in the workplace. Check the appropriate boxes to indicate the areas where students will develop the following skills (please note that all SCANS competencies do not apply to all courses):

RESOURCES

Managing Time: Selecting relevant goal-related activities, ranking them in order of importance, allocating time to activities, and understanding, preparing and following schedules.

Managing Money: Using or preparing budgets, including making cost and revenue forecasts; keeping detailed records to track budget performance, and making appropriate adjustments.

Managing Material and Facility Resources: Acquiring, storing, allocating, and distributing materials, supplies, parts, equipment, space or final products in order to make the best use of them.

INTERPERSONAL

Participating as Member of a Team: Working cooperatively with others and contributing to group's efforts with ideas, suggestions and effort.

Teaching Others New Skills: Helping others learn needed knowledge and skills.

Exercising Leadership: Communicating thoughts, feelings, and ideas to justify a position, encouraging, persuading, convincing or otherwise motivating an individual or group, including responsibly challenging existing procedures, policies or authority.

Negotiating: Working toward agreement that may involve exchanging specific resources or resolving divergent interests.

Working with Cultural Diversity: Working well with men and women and with people from a variety of ethnic, social, or educational backgrounds.

INFORMATION

Acquiring and Evaluating Information: Identifying a need for data, obtaining the data from existing sources or creating them, and evaluating their relevance and accuracy.

Organizing and Maintaining Information: Organizing, processing and maintaining written or computerized records and other forms of information in a systematic fashion.

Interpreting and Communicating Information: Selecting and analyzing information and communicating the results of others, using oral, written, graphic, pictorial, or multimedia methods.

Using Computers to Process Information: Employing computers to acquire, organize, analyze and communicate information.

SYSTEMS

Understanding Systems: Knowing how social, organizational and technological systems work and operating effectively with them.

Monitoring and Correcting Performance: Distinguishing trends, predicting impacts of actions on system operations, diagnosing deviations in the functioning of a system/organization, and taking necessary steps to correct performance.

Improving or Designs Systems: Making suggestions to modify existing systems in order to improve the quality of products or services and developing new or alternative systems.

TECHNOLOGY

Selecting Technology: Judging which sets of procedures, tools or machines, including computers and their programs, will produce the desired results.

Applying Technology to Tasks: Understanding overall intent and proper procedures for setting up and operating machines, including computers and their reprogramming systems.

Maintaining and Troubleshooting Equipment: Preventing, identifying, or solving problems with equipment, including computers and other technologies.

Section III: RELATIONSHIP TO COLLEGE PROGRAMS

1. THIS COURSE WILL BE AN APPROVED REQUIREMENT FOR AN APPROVED ASSOCIATE DEGREE OR CERTIFICATE PROGRAM: Yes

a. If yes, the course will be a requirement portion of the 'approved program' listed on the State Chancellor's Inventory of Approved Programs (approved programs can be found on the State Chancellor's Office website at https://misweb.cccco.edu/webproginv/prod/invmenu.htm)

2. GENERAL EDUCATION REQUIREMENTS FOR THE ASSOCIATE DEGREE STATUS:

a. Area Requested: None

Approval Date:

If applicable, provide an explanation of how the course meets the General Education parameters for one of the five general education areas - Natural Sciences, Social and Behavioral Sciences, Humanities, Language and Rationality, Health and Physical Education -- contained in Board Rule 6201.14 -General Education Requirements. http://www.laccd.edu/board_rules/documents/Ch.VI-ArticleII.pdf

b. Area Requested: None

Approval Date:

Section IV: ARTICULATION INFORMATION
(Complete in consultation with College Articulation Officer)

1. TRANSFER STATUS:

a. Transferable to the University of California: No

b. UC Approval Date:

c. Transferable to the California State University: No

d. College Approval Date: 10/4/16

2. GENERAL EDUCATION FOR TRANSFER:

  IGETC Certification

    a.  Area Requested:
    b.  Date Requested:
    c.  IGETC Approval Date:

If applicable, provide an explanation of how the course meets the appropriate General Education parameters, as defined in IGETC Certification Guidelines.

  CSU Certification

    a.  Area Requested:
    b.  Date Requested:
    c.  CSU Approval Date:

If applicable, provide an explanation of how the course meets the appropriate General Education parameters, as defined in CSU Certification Guidelines.

    a.  2nd Area Requested:
    b.  Date Requested:
    c.  IGETC Approval Date:

If applicable, provide an explanation of how the course meets the appropriate General Education parameters, as defined in IGETC Certification Guidelines.

    a.  2nd Area Requested:
    b.  Date Requested:
    c.  CSU Approval Date:

If applicable, provide an explanation of how the course meets the appropriate General Education parameters, as defined in CSU Certification Guidelines.

3. MAJOR REQUIREMENT FOR TRANSFER:

Will this course be articulated to meet lower division major requirements?:

List college/university and the majors:

CAN NUMBER: CAN SEQUENCE #:

CAN Approval -

Date requested: Date approved:

Section V: SUPPLEMENTAL COURSE INFORMATION

1. DEPT/DIVISION NAME: Life Sciences Department

2. DEPT/DIVISION CODE: 24

3. SUBJECT CODE: 923

4. SUBJECT ABBREVIATION: BIOTECH

5. RECOMMENDED MINIMUM QUALIFICATION AREA:

6. ABBREVIATION FOR TRANSCRIPTS: BIOTECH II

7. DEGREE CREDIT:

Indicate whether the course meet the 'standards for approval' for degree credit course set forth in Title 5, section 55002(a)(2), which requires the course to have a degree of intensity, difficulty, and vocabulary that the curriculum committee has determined to be at the college level: Degree Applicable

8. GRADING METHOD: LETTER GRADE

9. REPETITIONS: # of times repeated for credit: 0

If this course is repeatable, explain how repetition of this course meets Title 5, section 55041(c)(2)(B):

10. PRIOR TO TRANSFERABLE LEVEL:

This course attribute applies toEnglish, Writing, ESL, reading and mathematics courses ONLY. If applicable, indicate how many levels below the transferable level this course should be placed: Not applicable

11. CREDIT BASIC SKILLS:

Title 5, section 55000(j) defines basic skills as 'courses in reading, writing, computation, and English as a Second Language, which are designated as non-degree credit courses pursuant to Title 5, section 55002(b).': No

12. CROSS REFERENCE:

Is this course listed as equivalent in content to existing College/District courses in another discipline?: No

If Yes, list courses (documentation of cross-discipline agreement must be provided):

13. COURSE SPECIFICALLY DESIGNED FOR STUDENTS W/ DISABILITIES:

Title 5, section 56029 allows a course to be repeatable when continuing success of the students with disabilities is dependent on additional repetitions of a specific class. Is this course designated as an 'approved special class' for students with disabilities?: No

If yes, provide an explanation of how this course meets the requirements of Title 5, section 56029:

14. COOPERATIVE EDUCATION STATUS:

Title 5, section 55252 allows for two types of Cooperative Education: 1) General Work Experience Education -- i.e., supervised employment, which is intended to assist students in acquiring desirable work habits, attitudes and career awareness, which need not be related to the students' educational goals; or 2) Occupational Work Experience Education -- i.e., supervised employment, extending classroom based occupational learning at an on-the-job learning station, which is related to the students' educational or occupational goal. Is this course part of the college’s approved cooperative work experience education program?: No

15. COURSE CLASSIFICATION: Credit Course

Note: A course’s Classification, TOP Code and SAM code must be aligned – e.g., Courses with an 'Occupational' Course Classification must have an 'Occupational' TOP Code and a SAM Code of A, B, C, or D; courses that do not have an 'Occupational' Course Classification cannot have an Occupational TOP Code and must have an 'E' SAM Code. Courses coded as 'basic skills' in #11 should be coded 'Adult and Secondary Basic Skills.'

16. TOP CODE - (6 digits XXXX.XX): 0430.00

Course content should match discipline description in Taxonomy of Programs found at http://ecd.laccd.edu/TaxonomyOfPrograms.pdfcurriculum.htm

17. SAM CODE (Student Accountability Model): C

18. FUNDING AGENCY CODE:

19. STATE COURSE ID:

Section VI: APPROVAL STATUS

1. APPROVAL STATUS:

		Approval Date Of	Board Date	Requested Effective Semester	Approved Effective Semester
a.	New Course	College:	Board:	Effective Semester: Fall 2017	Effective Semester:
b.	Addition of Existing District Course	College: 10/4/16	Board: 12/7/16	Effective Semester:	Effective Semester:
c.	Course Change*	College:		Effective Semester:	Effective Semester:
d.	Outline Update	College:			Effective Semester:
e.	New Course	College:		Effective Semester:	Effective Semester:
f.	New Course	College:	Board:	Effective Semester:	Effective Semester:

* Changes to a course require the completion of a 'Course Change Request' form and approval by the college's Curriculum Committee.
In some cases districtwide approval is also required; see, Administrative Regulation E-65, section 3(c) for details.

Section VII: APPROVAL INFORMATION FOR NEW OR ADDED COURSES
(complete in consultation with Department Chair and the appropriate Academic Administrator)

1. ORIGINATOR: brownst

2. DEPARTMENT: 24

3. IF THIS IS A NEW COURSE, INDICATE HOW THE COLLEGE PLANS TO MEET THE EXPENSE OF THIS COURSE:

By additional funds. Describe:

additional budget or possibly regional CTE funds

By deleting courses from the college catalog and course database. List specific courses to be deleted:

By deleting sections of existing course. List courses and number of sections to be deleted:

FIRST YEAR: SECOND YEAR: THIRD YEAR:

By rotating sections of existing courses. List courses and number of sections to be rotated, as well as the semesters in which they will be offered:

4. IMPACT

IMPACT -- Will this course directly impact other course offerings and/or associate degree or certificate programs on campus? (If yes, briefly explain how)

This course will be part of a stackable certificate program and may be part of an Associate Degree program in Biotechnology.

5. METHOD OF SUPPORT

-- Indicate how the college plans to support the proposed course:

A. Additional staff -- List additional staff needed:

This class may require additional faculty which should be supported by additional budget or possibly regional CTE funds

B. Classroom -- List classroom type needed:

Molecular Biology Laboratory

C. Equipment -- List new equipment needed and indicate funding source for any new equipment:

Equipment purchased with CTE enhancement funds

D. Supplies- List supplies and indicate dollar value:

Plastic disposables, chemical reagent replacements. Initial supplies purchased with CTE enhancement funds. The total cost of supplies per class will be approximately $1000 per semester.

E. Library/Learning Resources- The course initiator shall consult with the College Librarian and review the college library, book, periodical, and electronic resource collections relevant to this course. List additional titles and resources to be considered for purchase as funding permits:

See Library Form addendum.

CERTIFICATION AND RECOMMENDATION

This course meets Title 5 requirements for Associate Degree applicable college credit towards an Associate Degree.

This course meets Title 5 requirements but does not satisfy the requirements for an Associate Degree applicable course.

We certify that the information and answers above properly represent this course.


Originator	Date
Department/Cluster Chairperson	Date
Articulation Officer	Date
Librarian	Date
Dean (if applicable)	Date
Curriculum Committee Chairperson	Date
Academic Senate President	Date
Vice President, Academic Affairs	Date
College President	Date

Section VIII: ADDENDA
(Uploaded Documents)

General	New Credit Form	111858_804_New_Credit_Course_Form - Biotech 003.doc
Prerequisite Document	Form for Biotech 002 prerequisite.	Prerequisite_Validation-Biotech 003.doc
General	Library form.	Library_Form - Biotech 003.pdf