Title: Learn: Introduction to Purification of GFP
Course: BTEC-1000-301-302-303-F21 > Section: Protein Purification

Module 3 Introduction to Purification of GFP

In the last module, you transformed bacteria with the gene for Green Fluorescent Protein. While GFP isn’t a medicine, it is a useful product that can be produced on an industrial scale, just like medicines. You are going to use the bacteria you transformed with pGLO to make and purify GFP in the lab. You’ll be doing small-scale production, with a simplified purification scheme - but the work you will do is analogous to what is done in industry.

Watch

Watch the videos on the next two pages  to see industrial-scale purification of GFP. It’s not important that you keep track of the valves, controllers, or details of the equipment, but pay attention to the overall workflow.

Watch

GFP Fermentation

The 4 Stages of Fermentation  (6 videos, each less than 2:00)