Title: Quiz: Testing Your Own DNA: Protocol and Vocabulary
Course: BTEC-1000-301-302-303-F21 > Section: DNA Analysis

Q: What is a SNP?
- A place in the DNA where a single base pair varies.
- A mutation that causes disease.
- A technique for making lots of copies of a particular region of DNA.
- A method of separating DNA by size.
A: A place in the DNA where a single base pair varies.

Q: What is the purpose of gel electrophoresis?
- To separate DNA by size.
- To identify mutations.
- To cut DNA at a particular sequence.
- To purify DNA.
A: To separate DNA by size.

Q: What do restriction enzymes do?
- Cut DNA at a particular sequence.
- Identify mutations.
- Separate DNA by size.
- Purify DNA.
A: Cut DNA at a particular sequence.

Q: What is the point of the saline mouthwash portion of the experiment?
- To collect cells and the DNA they contain for the analysis.
- To amplify the DNA for purposes of the analysis.
- To determine the ability of the taste buds to correctly identify the "taste" of saltiness.
- To analyze the ability of cheek cells to absorb phenylthiocarbamamide.
A: To collect cells and the DNA they contain for the analysis.

Q: What is the point of the "PCR" step?
- To amplify the TAS2R38 gene out of the overall genomic DNA of each person.
- To degrade the cheek cells and allow the genomic DNA to escape and be analyzed.
- To cut the genomic DNA into small pieces so it can be run on an agarose gel.
- To activate the HaeIII enzyme.
A: To amplify the TAS2R38 gene out of the overall genomic DNA of each person.

Q: What is HaeIII for?
- It is an enzyme that will cut the DNA if you have the sequence GGCC present in your TAS2R38 gene.
- It is an enzyme that is used to amplify the TAS2R38 gene.
- It is a gene present in DNA that allows mammals to taste bitter compounds.
- It is a dye that allows you to fluorescently stain DNA in an agarose gel.
A: It is an enzyme that will cut the DNA if you have the sequence GGCC present in your TAS2R38 gene.

Q: What SNP occurs in the gene that is responsible for PTC tasting that we are testing in this experiment?
- G to C substitution
- G to A substitution
- C to A substitution
- T to A substitution
- T to G substitution
A: G to C substitution

Q: Which DNA fragment will travel fastest in a agarose gel?
- 250 bp
- 500 bp
- 1000 bp
A: 250 bp