Title: Lab: pGLO Transformation
Course: BTEC-1000-301-302-303-F21 > Section: Genetic Engineering

Module 2 Lab: pGLO Transformation

Lab

The procedure for the lab starts on page 36 of the pGLO Student Manual. The procedure will take you approximately two hours, so schedule accordingly. On page 41, note that the bacteria need to grow overnight in the incubator. Ideally you would return the next day (at least 16 hours later) to see and analyze your results. If that’s not possible, write a note on the “Help, please!” board asking staff to move your plates to the refrigerator until you can return.

Note: at some points in the manual, it calls for you to use a sterile transfer pipette. Instead, you are to use the microptipettes with sterile pipette tips; these are far more accurate, and produce less plastic waste than the transfer pipettes.

Objectives

- Induce E. coli to make Green Fluorescent Protein.