Title: Industrial-scale GFP Purification
Course: BTEC-1000-301-302-303-F21 > Section: Protein Purification

Q: Which step can be considered “cell farming”?
- Fermentation
- Recovery
- Purification
A: Fermentation

Q: What is lag phase?
- The period in which cells are adapting to a new environment, before they start growing.
- The period in which cells are continually dividing.
- The period in which equal numbers of cells are dying and dividing.
- The period in which cells are dying.
A: The period in which cells are adapting to a new environment, before they start growing.

Q: What does media mean in the context of GFP production and purification?
- The material that provides nutrition to cells.
- The data collected by the bioreactor.
- Newspapers, TV, radio, etc.
- The liquid collected as waste after centrifugation.
A: The material that provides nutrition to cells.

Q: In the video, IPTG is added to cause the cells to turn on production of GFP. This is because they are expressing GFP off a different plasmid than we are; theirs has control elements that cause GFP to be turned on by IPTG. What chemical causes GFP to be turned on in the cells you transformed?
- Arabinose
- Ampicillin
- Glucose
- Yeast extract
A: Arabinose

Q: Which step happens first during recovery?
- A centrifuge separates the cells from excess liquid.
- The cells are homogenized.
- The cells are washed.
- The lysate is filtered.
A: A centrifuge separates the cells from excess liquid.

Q: What does “elution” mean?
- Release of GFP from the beads of a column.
- Breaking cells open.
- Filtering out extra salts.
- Making the GFP more concentrated.
A: Release of GFP from the beads of a column.

Q: Why is salt important in hydrophobic interaction chromatography?
- The salt allows GFP to bind to the beads.
- The salt is required for bacterial growth.
- The salt is required for GFP to create the glow.
- The salt helps make the GFP more concentrated.
A: The salt allows GFP to bind to the beads.