Title: Column purification of the PCR
Course: BTEC-1100-301-302-303-F21 > Section: Module 3 - Polymerase Chain Reaction

Following the PCR reaction, you will be taking your PCR product and using it in the next step.  Please read the purification manual from Zymo Research found here:

https://files.zymoresearch.com/protocols/_d4003t_d4003_d4004_d4013_d4014_dna_clean_concentrator_-5.pdf

The short protocol you will use in the lab is found on page 4 of this manual.  At step 5, elute the DNA with 15 ul of elution buffer.

Remember: The next step is to come into the lab to practice with the materials, view a demonstration, or to even just ask questions. Also, if you are ready to take the lab final, please inform your instructor. The lab final will be performed by yourself and will be graded with a rubric.

Learning Objectives:

- Purify the polymerase chain reaction DNA using columns.