Title: Running a gel
Course: BTEC-1100-301-302-303-F21 > Section: Module 3 - Polymerase Chain Reaction

You should be familiar already with the steps for running a gel to see if your PCR and purification were successful.

Make a 0.8% agarose gel by adding 0.4 grams of agarose to 50 ml of 1X TAE. After dissolving the gel add 5 μl of 10 mg/ml ethidium bromide for visualization.

You will need to obtain the concentration of your purified PCR using the spectrophotometer and run between 100-200 ng of your product to visualize on the gel.  Don’t forget to run 5 μl of the DNA ladder, generally we use a 1 kB ladder a shown below.

Remember: The next step is to come into the lab to practice with the materials, view a demonstration, or to even just ask questions. Also, if you are ready to take the lab final, please inform your instructor. The lab final will be performed by yourself and will be graded with a rubric.