Title: BTEC-1100-301-302-303-F21

Orientation Module
  Welcome
  How Competency-based Education Works
  Get to Know Your Course
  Using the Open Lab
  Learning and Technical Support
  Netiquette
  Introduce Yourself
  Any Questions? (optional)
Module 1 - Calculations and Measurements
  Introduction
  A Review of Scientific Notation
  Scientific Notation: Practice Quiz #1
  The Metric System
  Metric System Prefixes
  Measuring Volume and Mass
  Dimensional Analysis
  Concentrations by mass/volume and %
  Calculating Percentages: Practice Quiz #2A (required)
  Calculating Percentages: Practice Quiz #2B (optional)
  Calculating Percentages: Practice Quiz #2C (optional)
  Moles and Molarity
  Calculating Molarity: Practice Quiz #3A (required)
  Calculating Molarity: Practice Quiz #3B (optional)
  Calculating Molarity: Practice Quiz #3C (optional)
  Concentrated Stock Solutions
  The Dilutions Equation: C1V1 = C2V2
  Dilutions Equation: Practice Quiz #4A (required)
  Dilutions Equation: Practice Quiz #4C (optional)
  Dilutions Equation: Practice Quiz #4B (optional)
  Serial Dilutions
  Serial Dilutions and Spectrophotometry
  Calculations and Measurements Exam#1
Module 2 - Molecular Biology
  Introduction to Module - Molecular Biology
  Genomic DNA
  Nucleic Acids - Structure and Function
  DNA Replication
  Transcription
  Translation
  The Central Dogma
  The Importance of Plasmids
  Our Plasmid
  Exam 2
Module 3 - Polymerase Chain Reaction
  Keeping a Laboratory Notebook
  BTEC 1100 Project Flow Chart
  Introduction to the PCR Reaction
  Steps of PCR - review
  Reagents in PCR - review
  Using the NCBI database to find a gene sequence
  Video Tutorial on NCBI
  Designing Primers
  Video Tutorial on Primer Design
  Assignment: Designing Primers
  Amplifying a specific gene through PCR
  Lab #2: PCR Lab
  Column purification of the PCR
  Lab #3: Column purification of the PCR
  Running a gel
  Lab #4: Run a Gel
Module 4 - Applied Molecular Biology
  Introduction to Recombinant DNA
  Ligation Independent Cloning
  Lab #1: Ligation Independent Cloning
  Transforming Bacteria with the LIC Reaction
  Lab #2: Transform Bacteria
  Screening Bacteria for Recombinant bglA-pLATE31 Plasmid
  Lab #3: Screening Bacteria for Recombinant bglA-pLATE31 Plasmid
  Isolating recombinant plasmid DNA
  Lab #4: Isolating recombinant plasmid DNA
  Restriction Enzymes
  Restriction Digest Verification of Recombinant bglA-pLATE31 Plasmid-2
  Analyzing Sequencing Data
  Tutorial on DNA Sequence Analysis
  Lab #5: Analyze Sequencing Data
  Exam 3
  Final Exam