Title: Introduction to the PCR Reaction
Course: BTEC-1100-301-302-303-F21 > Section: Module 3 - Polymerase Chain Reaction

In this module you will be learning about the Polymerase Chain Reaction (PCR) in depth.  You have already been introduced to PCR in your other class but now you will learn all the details of the method so that you will be trained to set up and perform PCR experiments in the future.  As you have already learned the PCR reaction has revolutionized every aspect of biology and has had a huge influence on society itself.  The importance of this technique cannot be overstated.

Learning Objectives:

- Explain the steps of a polymerase chain reaction.
- Describe the reagents in a polymerase chain reaction.
- Explain how to design primers for a polymerase chain reaction.
- Find a given gene sequence in the NCBI database.
- Design primers for a polymerase chain reaction.
- Conduct a polymerase chain reaction to isolate a specific gene.
- Purify the polymerase chain reaction DNA using columns.
- Run a gel.