Title: Our Plasmid
Course: BTEC-1100-301-302-303-F21 > Section: Module 2 - Molecular Biology

The plasmid we will be using to clone our gene into is called pLATE 31.  It is manufactured as a vector for cloning of genes and expression of proteins from the recombinant DNA.

From this map you can see all of the DNA sequences needed to express a protein from a cloned gene.  There is an origin of replication (rep) needed for replication, a T7 promoter (PT7) and T7 terminator (T T7) needed for transcription, and a ribosomal binding sequence (RBS) needed for translation.  With all the machinery in place, once our gene is correctly cloned into pLATE31 we will be able to produce a protein product.