Title: Assignment: Designing Primers
Course: BTEC-1100-301-302-303-F21 > Section: Module 3 - Polymerase Chain Reaction

Using the NCBI database search for any gene.  You can input practically anything and you will find an entry in the database.  For instance, I searched my initials, CCR and found this in the GENE database:

https://www.ncbi.nlm.nih.gov/gene/548269 (Links to an external site.)Links to an external site.

You could also search for anything that may interest you.  I searched for “male pattern baldness” and I was able to pull up 4 hits in the GENE database including this one:

https://www.ncbi.nlm.nih.gov/gene/367 (Links to an external site.)Links to an external site.

Once you have found something interesting answer the following questions in a word document:

1) What is the gene description and abbreviation of the gene?

2) What species does the sequence data come from?

3) What is the size of the gene and the protein it codes for?

4) What does the protein do?

5) Find the FASTA sequence and copy it into your word file.

Finally, using the FASTA sequence  design forward and reverse primers that will enable you to amplify the gene sequence.  Aim for a 55-60°C Tm for each of the primers.  I usually use the Biotools website from Northwestern to find my melting temperatures and other useful information.

Link

Please submit your answers by e-mail to charles.rettberg@slcc.edu

Have Fun!