Title: Isolating recombinant plasmid DNA
Course: BTEC-1100-301-302-303-F21 > Section: Module 4 - Applied Molecular Biology

Based on the results from the previous section you should have several positive clones.  We will now isolate the recombinant plasmid from one of those clones in order to sequence the DNA using a very common molecular biology technique called a plasmid miniprep.

First you will need to grow up several milliliters of bacterial culture for use as the starting material.  You will use your knowledge of microbiology techniques to start that culture in LB media containing 50 μg/ml ampicillin.  Following overnight growth at 37°C you will use the following manual.  Another resource can be found here.

After isolating your plasmid you will want to take a spectrophotometer reading to determine your DNA concentration.