Title: Learn: Maximal Velocity and the Michaelis-Menten plot
Course: BTEC-2020-301-302-303-F21 > Section: Enzyme Kinetics

Module4 Maximal Velocity and the Michaelis-Menten plot

Watch

To help you understand what’s going on in enzyme kinetics studies, watch the video below of a hands-on demonstration of enzyme kinetics. A student’s hand serves as the “active site” and we stipulate that each active site can bind only one substrate at a time. A microcentrifuge tube in the original box is substrate, while a microcentrifuge tube moved to the other box by the enzyme/hand is product. The student demonstrates the effect of increasing substrate concentration on the formation of product.

Watch

Next, watch the video below to learn more about the enzyme kinetics experiments you’ll be performing in the lab. For now, you’ll use trypsin, a well-characterized enzyme.