Title: Using the Open Lab
Course: BTEC-2020-301-302-303-F21 > Section: Course Orientation

All students who work in the lab must clean up after themselves, participate in community chores, and maintain an environment conducive to experimentation and learning for all who use the learning lab.

The learning lab is open during the semester:

Monday - Thursday, 10am - 8pm

Saturday, 8am - 2pm

The lab is closed for semester breaks and College holidays.

To ensure space is available in the open lab, you must reserve your selected time through biotech.youcanbook.me.

Instructions for booking are available in the Youcanbook instructions.

For an accessible booking arrangements, contact Shauna.Gordon@slcc.edu. or call (801) 957-2851.

Module | Lab task | Estimated lab time
1 | none | 0:00
2 | Pour SDS-PAGE gels | 1:30
 | Run and stain SDS-PAGE gels | 1:00 (plus hour or more incubation)
 | Destain SDS-PAGE gels | 0:05 (plus overnight incubation)
 | Bradford assay and calculate amounts to load | 2:30
 | Determine appropriate amounts of protein to load via SDS-PAGE | 4:00 (could break up over two days)
3 | Size exclusion chromatography | 2:00
 | Dialysis | 0:15 (plus overnight incubation)
4 | Trypsin kinetics, varied [enzyme] | 2:00
 | Trypsin kinetics, varied [substrate], +/- inhibitor | 2:30
5 | BglA expression | 3:00 – 6:00 (depends on cell growth; also requires a culture to have been started the previous day)
 | Check induction via SDS-PAGE, start Western blot (transfer to membrane) | 3:00 (plus 1 hour or overnight incubation)
 | Evaluate expression via SDS-PAGE and finish western blot | 4:00 (could break up over two days)
 | Make lysis, purification buffers for BglA | 2:00
 | Lyse cells, spin down, and freeze for purification | 2:00
 | BglA FPLC purification, set up dialysis | 3:30
 | Evaluate BglA presence in fractions via SDS-PAGE | 4:00 (could break up over two days)
 | Bradford assay to determine BglA concentration | 2:00
 | BglA kinetics | 6:00 (could break up over multiple days)

Remember: experiments do fail sometimes, so it's possible you may need to repeat one or more of labs. Also keep in mind that you can do more than just lab work in the lab; this is where you can ask faculty questions, and talk about the online course materials.