Title: Learn: Introduction to BglA Kinetics Project
Course: BTEC-2020-301-302-303-F21 > Section: Final Project and Exam

Module 5 Introduction to BglA Kinetics Project

So far in this course, you have learned several ways to analyze and purify proteins. Now, it’s time to put these skills together in service of a goal: to express, purify, and determine the turnover rate of the enzyme BglA under standard conditions, and an alternate condition of your own choosing. (Recall the BglA encodes beta-glucosidase; you cloned the BglA gene from Bacillus halodurans into a plasmid, and transformed E. coli with the plasmid.)

There is one new topic and skill for you to learn before the project begins: protein expression. Continue to the next page to learn about it.