Title: Learn: Introduction to Enzyme Kinetics
Course: BTEC-2020-301-302-303-F21 > Section: Enzyme Kinetics

Module4 Introduction to Enzyme Kinetics

Like a watch wrapped around a wrist, a special enzyme encircles the double helix to repair a broken strand of DNA. Without molecules that can mend such breaks, cells can malfunction, die, or become cancerous.
Courtesy of Tom Ellenberger, Washington University School of Medicine in St. Louis, and Dave Gohara, Saint Louis University School of Medicine
NIH funding from: National Institute of General Medical Sciences (NIGMS)

Introduction

In this module, you’ll learn about enzyme kinetics and how to measure an enzyme’s rate of turnover.

After completing this module you should be able to demonstrate Competency  5: The student determines the turnover rate of an enzyme in particular conditions.

You will watch a series of narrated PowerPoints that explain enzyme kinetics and then you will perform your second milestone lab assignment and take a practice quiz to help you determine your progress toward these objectives that support the competency.

Objectives

After completing this module you should be able to demonstrate Competency  5: The student determines the turnover rate of an enzyme in particular conditions.

- Explain the purpose of enzyme kinetics.
- Measure an enzyme’s rate of reaction with various substrate concentrations.
- Create a Michaelis-Menten plot.
- Determine maximal velocity from a Michaelis-Menten plot.
- Explain why enzymes have maximal velocity.
- Calculate turnover rate from maximal velocity and enzyme concentration.
- Explain how the enzyme inhibitor binding site affects turnover rate.