Title: Lab: Purify Small Molecules Via Chromatography
Course: BTEC-2020-301-302-303-F21 > Section: Chromatography

Module3 Chromatography and Dialysis

Lab

In the lab, you will use size exclusion chromatography to separate two molecules: blue dextran (MW ~ 2,000,000) and phenol red (MW 354). These are not proteins, but dyes. If we were actually separating proteins, we would do the entire procedure in the 4 degree cold room, as cold temperatures protect proteins from denaturing. By doing this activity with dyes, you will be able to see the separation in real time - and you won’t need a winter coat. After chromatography, you will also learn about and practice dialysis. Download the protocol below.

Chromatography and Dialysis Protocol

Objectives

- Purify small molecules via chromatography