Title: Learn: Turnover Rate and the Enzyme Inhibitor Binding Site
Course: BTEC-2020-301-302-303-F21 > Section: Enzyme Kinetics

Module4 Turnover Rate and the Enzyme Inhibitor Binding Site

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As you can guess from the name, “soybean trypsin inhibitor” tends to slow the reaction of the enzyme trypsin. But how does it slow it down? How can we determine what it does? Enzyme kinetics studies can help us understand exactly how an enzyme inhibitor works. Pharmaceuticals are often enzyme inhibitors, which . Non-steroidal anti-inflammatory drugs (NSAIDs), like aspirin, ibuprofen and naproxen, work by inhibiting the activity of cyclooxygenase enzymes (COX-1 and/or COX-2). The COX enzymes make prostaglandins, which promote pain, fever, and inflammation. By inhibiting the COX enzymes, you can reduce the amount of prostaglandins made, and thereby reduce pain, fever, and inflammation.

In your experiment, you will determine the mechanism of inhibition employed by soybean trypsin inhibitor. Watch the video below to learn about the experiment, and different types of enzyme inhibitors.

Jean's PPT when ready