Title: Quiz: Enzyme Kinetics
Course: BTEC-2020-301-302-303-F21 > Section: Enzyme Kinetics

Q: In a theoretical situation with infinite substrate, what could be reached?
- V_max
- k_cat
- K_m
- Hyberbolic kinetics
A: V_max

Q: What is another name for turnover number?
- V_max
- k_cat
- K_m
- Hyperbolic kinetics
A: k_cat

Q: In a kinetic study of the enzyme peptidase, 2 mM of peptidase had a maximal velocity of 12 mM/sec. What is the kcat for this enzyme under these conditions?
- 6/sec
- 6 mM/sec
- 24/sec
- 24 mM/sec
A: 6/sec

Q: You are performing enzyme kinetics studies on the enzyme lipase. You find a lipase inhibitor that works well at low substrate concentrations without affecting the enzyme’s maximal velocity. Where does the lipase inhibitor bind to the enzyme?
- Active site
- Allosteric site
- Lipid bilayer
- Peptidoglycan bilayer
A: Active site

Q: What is the approximate V_max for the enzyme that produced the data below?
- 13 uM/sec
- 13 uM
- 10uM/sec
- 10 uM
A: 13 uM/sec

Q: Which question could be answered using an enzyme kinetics study?
- What temperature is optimal for the enzyme?
- What is the affinity of an enzyme for a secondary antibody?
- How many kilodaltons is an enzyme?
- What is the concentration of an enzyme?
A: What temperature is optimal for the enzyme?