Title: Learn: Analyzing SDS-PAGE Results
Course: BTEC-2020-301-302-303-F21 > Section: Protein Gels

Module2 Analyzing SDS-PAGE Results

After the staining and destaining procedure, blue bands of protein should be visible in the gel. The gel may be photographed using white light, or dried between sheets of cellophane.

The migration of bands of your unknown proteins can be compared to the bands of the ladder, which is composed of proteins of known molecular weights.

This procedure is analogous to to the way you have determined size of DNA fragments on agarose gels in the past.