Title: Contamination Screening through PCR
Course: BTEC-2030-301-302-303-F21 > Section: Cell Culture

We use PCR to identify if we have a type of bacterial infection called mycoplasma. Mycoplasma are unique small bacteria that are often antibiotic resistant and difficult to culture in bacterial media. We use PCR with specific primers designed to amplify sequences unique to the mycoplasma to determine if the bacteria is present.

You will use a kit to conduct a PCR contamination screening on your cells. Ask your instructor for the kit before you get started.

Learning Objectives:

- 1.8 - Screen cultured cells for contamination through visual observation and PCR.