Title: TurboFect
Course: BTEC-2030-301-302-303-F21 > Section: Cell Culture

TurboFect is a transfection reagent is an of lipid-based chemical that is used to gently transfer your desired DNA vector into a eukaryotic cell. It works much the same way as vesicles that you have learned about in biology class. We incubate the TurboFect with our desired DNA and the DNA is trapped in the interior of the vesicle.  The vesicle then merges with the plasma membrane of the cell releasing the DNA into the interior of the cell.

Learning Objectives:

- 1.6 - Transfect cells for fluorescence.