Title: Observing Fluorescence in Eukaryotic Cells
Course: BTEC-2030-301-302-303-F21 > Section: Cell Culture

Now that you have transfected your mammalian cell, you should have a glowing green cell. It will look something like this:

To determine if you’ve transfected your cell correctly, you’ll need to observe your cell with a fluorescent microscope. This microscope works the same way as a phase contrast microscope except that instead of visible light to observe cells, the microscope shines UV light on the cells to make them fluoresce.

Chuck shows you how to set up the microscope for observation of your cells in this video.

Learning Objectives:

- 1.7 - Observe cells using a fluorescence microscope.