Title: qPCR
Course: BTEC-2200-301-302-303-F21

qPCR videos

Introduction to qPCR

Overview of qPCR (Links to an external site.)Links to an external site.

Note that the video above refers to C_q values; C_q (Cycle quantitation) is also known as C_T (Cycle threshold).

SYBR Green and melt curves

Finding Multiple Melt-Curve Peaks When Using SYBR® Green in Real-Time PCR -- Ask TaqMan®: Ep. 8 (Links to an external site.)Links to an external site.

How TaqMan works

How TaqMan Works -- Ask TaqMan® Ep. 13 by Life Technologies (Links to an external site.)Links to an external site.

Real-Time PCR Thresholds and Where to Place Them

Real-Time PCR Thresholds and Where to Place Them -- Ask TaqMan®: Ep. 6 (Links to an external site.)Links to an external site.

The Purpose of Rox

The Purpose of ROX™ in Real-Time PCR -- Ask TaqMan®: Ep. 7 (Links to an external site.)Links to an external site.

Getting Reliable Data in qPCR

Getting Reliable Data in qPCR - Ask TaqMan #22 (Links to an external site.)Links to an external site.

Real-Time PCR Applications

Real-time PCR Applications -- Ask TaqMan® Ep. 17 by Life Technologies (Links to an external site.)Links to an external site.