Freezing and Thawing Human Embryonic Stem Cells

Lia Kent, of Stemgent's Research and Development team, has created this video to demonstrate "the proper technique for rapidly thawing hES cells from liquid nitrogen stocks, plating them on mouse embryonic feeder cells, and slowly freezing them for long-term storage." The video is also accompanied by protocols for thawing and freezing hES cells, discussion, materials, references, a forum for comments, and a PDF of the full text.