Fluorescent Tagging of Liposomes Lab

This resource, provided by the Nanotechnology Applications and Career Knowledge Support Center (NACK Center), includes a lab guide and corresponding videos that walk through the process of examining "the liposome as a carrier of fluorescent tags used in cancer identification." Throughout the lab, students will create liposomes from raw materials, which will then be "tagged, extruded to a proper size, filtered, and characterized." The 20-page lab guide includes objects, equipment needed, a 24-step lab procedure, and an explanation of results. Additionally, the lab guide provides background on manufacturing and tagging, extrusion, and liposome filtering. Included are nine demonstration videos that correspond with different sections of the lab as noted by the lab guide.

Below is a list of the files contained within the .zip attachment. The size of each file is included in parenthesis.

Fluorescent Tagging of Liposomes_Videos (9 files, 276 MB)

• Hydration of Sephadex G-50 and Liposome Samples (01-NACK-Hydration_of_Sephadex_G-50_and_Liposome_Samples.mp4 12.6 MB)
• Avanti Mini-Extruder Preparation Steps (02-NACK-Avanti_Mini-Extruder_Preparation_Steps.mp4 24.2 KB)
• Extrusion of Sample A (03-NACK-Extrusion_of_Sample_A.mp4 29.3 MB)
• How to Clean the Syringe and Extruder Parts (04-NACK-How_to_Clean_the_Syringe_and_Extruder_Parts.mp4 5.12 MB)
• Gel Chromatography Column Setup (05-NACK-Gel_Chromatography_Column_Setup.mp4 15.1 MB)
• Column Chromatography of Sample A (06-NACK-Column_Chromatography_of_Sample_A.mp4 12.3 MB)
• Column Chromatography of Sample B (07-NACK-Column_Chromatography_of_Sample_B.mp4 12.1 MB)
• The Zetasizer Nano Particle Chacterization System (08-NACK-The_Zetasizer_Nano_Particle_Chacterization_System.mp4 87.2 MB)
• The New Zetasizer Nano ZSP (09-NACK-The_New_Zetasizer_Nano_ZSP.mp4 78.2 MB)