Multicolor Antibody Staining of T Cells

This 13-page resource, from Frederick Community College is a standard operating procedure (SOP) for multicolor panel antibody staining of T Lymphocytes. The SOP outlines the purpose, scope, definitions, references, safety measures, responsibilities, materials and equipment involved in the procedure. Step-by-step procedures are provided for assessing the presence of a panel of antibodies on the surface of CD3 Jurkat cells to understand lymphocyte expression profiles accurately.

Procedure steps include: confirming the cytometer is on and calibrated, preparing the sample, compensation control preparation, sample staining, setting the acquisition parameters on the cytometer, creating sample gating strategy on the cytometer, sample acquisition, data analysis, and exporting the data. Detailed steps are included.