Basic Biotechnology Kit: Restriction Enzyme Analysis

Restriction mapping is a cornerstone of modern-day biotechnology. During this three-day exercise, students will digest samples of DNA with three different restriction enzymes. The DNA samples will be electrophoresed and the resulting fragments photographed under and ultraviolet transilluminator. Attached readings and activities will help explain the process of restriction enzyme analysis and how it is used in the DNA fingerprinting procedure. This 17-page pdf contains teacher information for presenting the activity, the complete procedure of the laboratory, and two student activities.