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The discovery of enzymes that could cut and paste DNA made genetic engineering possible. Restriction enzymes, found naturally in bacteria, can be used to cut DNA fragment at specific sequences, while another enzyme, DNA ligase, can attach or rejoin DNA fragments with complementary ends. This...

Stanley Cohen and Herbert Boyer's historic experiment used techniques to cut and paste DNA to create the first custom-made organism containing recombined or 'recombinant' DNA. Cohen and Boyer inserted the recombinant DNA molecule they created into E. coli bacteria by means of a plasmid, thereby...

DNA transformation is a naturally occurring but rare event in which DNA can be transferred into bacteria. In 1970, Morton Mandel and Akiko Higa discovered a way to make E. coli more 'competent' for transforming foreign DNA. Their calcium chloride method is widely used today to obtain high-efficiency...

This resource from Northeast Biomanufacturing Center and Collaborative (NBC2) provides information materials on downstream processing, such as textbook chapters, standard operating procedures, interactive learning resources, and videos. Resource titles include Affinity Chromatography of HSA SOP,...

This two page PDF, created by Northeast Biomanufacturing Center and Collaborative, discusses the key functions and tasks of a manufacturing technician (downstream). The document focuses mostly on: the work compliance with both EH&S and cGMPS, the cleanliness and maintenance of production areas,...

This video, provided by Digital World Biology, is from a series of tutorial videos for iCn3D, a web-based three-dimensional visualization and structural analysis platform for molecular structures. During this video, presenter Sandra Porter offers step-by-step instructions on how to view and analyze...

This video, provided by Digital World Biology, is from a series of tutorial videos for iCn3D, a web-based three-dimensional visualization and structural analysis platform for molecular structures. In this video, presenter Sandra Porter discusses using the protein Basic Local Alignment Search Tool...

This video, provided by Digital World Biology, is from a series of tutorial videos for iCn3D, a web-based three-dimensional visualization and structural analysis platform for molecular structures. This tutorial builds on the previous video in the series: Drug Discovery 2: Using Protein BLAST For...

In this video, created by Support Center for Microsystems Education (SCME), viewers can watch an animation on the dry thermal oxidation process. This animation "illustrates the chemistry of a dry thermal oxidation process that is used to grow silicon dioxide (SiO2) on a silicon (Si) wafer. In a dry...

This webinar, published by InnovATEBIO, is the third in a webinar series that presents on CRISPR gene editing. In the video, Eric B. Kmiec and John V. McDowell demonstrate how educators can develop a program for gene editing, provide information on new initiatives, and present course examples....